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Universal Plus mRNA-Seq with NuQuant

Universal Plus mRNA-Seq kit with NuQuant® Library Quantification method is a novel and streamlined library preparation solution for mRNA sequencing on Illumina sequencers. This RNA-Seq library preparation kit is integrated with NuQuant, a proprietary library quantification method for efficient and accurate quantitation of Next-Generation Sequencing (NGS) libraries. The NuQuant method eliminates the need for time-consuming or inaccurate library quantification methods like qPCR, fluorometry (e.g. Qubit®) or microfluidic electrophoresis (e.g. Bioanalyzer®) allowing RNA-Seq libraries to be constructed and quantified in a single day.

Universal Plus mRNA-Seq kit with NuQuant® Library Quantification method is a novel and streamlined library preparation solution for mRNA sequencing on Illumina sequencers. This RNA-Seq library preparation kit is integrated with NuQuant, a proprietary library quantification method for efficient and accurate quantitation of Next-Generation Sequencing (NGS) libraries. The NuQuant method eliminates the need for time-consuming or inaccurate library quantification methods like qPCR, fluorometry (e.g. Qubit®) or microfluidic electrophoresis (e.g. Bioanalyzer®) allowing RNA-Seq libraries to be constructed and quantified in a single day.

Key features of this mRNA-Seq library preparation kit include:

  1. Up to 192 Unique Dual Index (UDI) pre-plated adaptors with molecular tags
  2. Fewer bead purification steps for an efficient and streamlined library preparation workflow for any sample type
  3. Broad dynamic input range from 1 μg to as low as 10 ng, enables Illumina sequencing of previously inaccessible samples
  4. Eliminate adaptor dimers without adaptor titration regardless of sample input using the DimerFree® technology
  5. Accurately quantify library concentrations (in nM) with a simple fluorescent measurement using NuQuant library quantification method at no additional cost
  6. Remove unwanted reads from sequencing data using AnyDeplete mediated transcript depletion after library preparation (Learn more in our Application Note)

NuQuant app for Qubit can be downloaded here on Github.

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