Accel-NGS^® Methyl-Seq DNA library Kit

The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA recovery of bisulfite-converted samples and constructs libraries that accurately represent sample composition.
The Accel-NGS Methyl-Seq workflow maximizes DNA recovery through a post-bisulfite library preparation, utilizing a highly efficient adapter attachment that is compatible with single- stranded, bisulfite-converted DNA. Library yields from this kit are up to 100x greater than those from methods that bisulfite convert after library construction. Additionally, the template- independent adapter attachment chemistry of the Accel-NGS Methyl-Seq Kit provides a more complete, less biased library as observed from comprehensive methylome coverage by Whole Genome Bisulfite Sequencing (WGBS).

Features

High recovery of input DNA

Low bias library preparation

Simple, 2-hour library prep

Compatable with Illumina® platforms

Minimal PCR cycles required

-  4 cycles for 100 ng

-  7 cycles for 10 ng

-  11 cycles for 1 ng

-  14 cycles for 100 pg

Applications

WGBS

Reduced Representation Bisulfite Sequencing (RRBS)

Detecting genome-wide methylation in 5 ng of cfDNA

Hybridization capture using NimbleGen^TM SeqCap^TM Epi Enrichment System

Bisulfite-converted DNA enriched by MeDIP, ChIP or other methods

Order Information

Product Information	Catalog No.
Accel-NGS Methyl-Seq DNA Library Kit,24 rxn	30024
Accel-NGS Methyl-Seq DNA Library Kit,96rxn	30026