- Accel-NGS® Methyl-Seq DNA library Kit
The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA recovery of bisulfite-converted samples and constructs libraries that accurately represent sample composition.
Accel-NGS® Methyl-Seq DNA library Kit
The Accel-NGS Methyl-Seq DNA Library Kit maximizes DNA
recovery of bisulfite-converted samples and constructs
libraries that accurately represent sample composition.
The Accel-NGS Methyl-Seq workflow maximizes DNA recovery through a post-bisulfite library preparation, utilizing a highly efficient adapter attachment that is compatible with single- stranded, bisulfite-converted DNA. Library yields from this kit are up to 100x greater than those from methods that bisulfite convert after library construction. Additionally, the template- independent adapter attachment chemistry of the Accel-NGS Methyl-Seq Kit provides a more complete, less biased library as observed from comprehensive methylome coverage by Whole Genome Bisulfite Sequencing (WGBS).
High recovery of input DNA
Low bias library preparation
Simple, 2-hour library prep
Compatable with Illumina® platforms
Minimal PCR cycles required
- 4 cycles for 100 ng
- 7 cycles for 10 ng
- 11 cycles for 1 ng
- 14 cycles for 100 pg
Reduced Representation Bisulfite Sequencing (RRBS)
Detecting genome-wide methylation in 5 ng of cfDNA
Hybridization capture using NimbleGenTM SeqCapTM Epi Enrichment System
Bisulfite-converted DNA enriched by MeDIP, ChIP or other methods
Accel-NGS Methyl-Seq DNA Library Kit,24 rxn
Accel-NGS Methyl-Seq DNA Library Kit,96rxn
The Accel-NGS 1S Plus DNA Library Kit is designed for Illumina® platforms. Utilizing Swift Biosciences’ innovative technology, this kit allows DNA library construction from single-stranded DNA (ssDNA), as well as double-stranded DNA (dsDNA), which is nicked, damaged, or contains short fragments.查看详细